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. Author manuscript; available in PMC: 2024 Sep 11.
Published in final edited form as: Curr Biol. 2023 Aug 7;33(17):3660–3668.e4. doi: 10.1016/j.cub.2023.07.043

Figure 2. Sleep-promoting effects of 23E10 neurons map to the VNC.

Figure 2.

(A) Thermogenetic activation of 23E10 neurons at 32° C from ZT0–6 promotes sleep and is blocked by tsh-Gal80. N≥42 for each genotype. (B) Sleep in A is rapidly reversible in the presence of strong mechanical agitation at ZT1. N≥42 for each group. (C) Tsh-Gal80 does not reduce the number of dFB cell bodies in the brain that are labeled by 23E10>GFP. N≥24 for each group. (D-E) Representative images of dFBs in 23E10>GFP brains in the absence (D) vs presence (E) of tsh-Gal80. (F-G) Representative images of 23E10>GFP VNCs in the absence (F) vs presence (G) of tsh-Gal80. All images are max projections of whole mount preparations. Data are presented as mean ± SEM. **** p<.0001 and ns = not significant by one-way ANOVA. Scale bars = 40 um. See also Figure S1.