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. Author manuscript; available in PMC: 2024 Sep 11.
Published in final edited form as: Curr Biol. 2023 Aug 7;33(17):3660–3668.e4. doi: 10.1016/j.cub.2023.07.043

Figure 5. Sand and AstA transcripts exhibit little to no expression in 23E10 neurons.

Figure 5.

(A) Whole-brain image with multiplexed labeling of 23E10-driven GFP by IHC and Sand transcript by RNAscope. (B-D) Rotated volumetric reconstruction (.25 um slices) of inset in A showing 23E10-labeled cell bodies by IHC (B), labeling of Sand mRNA by RNAscope (C), and a merge of both labels (D). (E) Volume projection of whole mount brain with multiplexed labeling of 23E10-driven GFP by IHC, AstA protein by IHC and AstA transcript by RNAscope. (F-I) Rotated volumetric reconstruction (.25 um slices) of inset in E showing 23E10-labeled cell bodies by IHC (F), labeling of AstA protein by IHC (G), labeling of AstA mRNA by RNAscope (H), and a merge of all three labels (I). (J) Average percentage of 23E10 cell bodies per hemisphere that show one or two puncta of each mRNA. N=36 (AstA) and N=71 (Sand) total GFP-positive cells analyzed per group. See also Figure S4.