Key Resources Table

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-V5	Thermo Fisher	PA1993
Rabbit anti-HA	Rockland	RL600-401-384
Rat anti-HA	Roche	11867423001
Rabbit anti-GFP	Invitrogen, Life Technologies	A11122
Mouse anti-AstA	DSHB	5F10-c
Mouse anti-nc-82	DSHB	Nc-82-c
Goat anti-rabbit Alexa Fluor 488	Thermo Fisher	A11008
Goat anti-mouse Alexa Fluor 633	Thermo Fisher	A11009
Goat anti-mouse-HRP	VWR	95959–740
Goat anti-rabbit-HRP	VWR	95959–088
Bacterial and virus strains
Biological samples
Chemicals, peptides, and recombinant proteins
Vectashield	Vector labs	H-1000
Critical commercial assays
Multiplex Fluorescent Detection Kit v2	Advanced Cell Diagnostics	323110
RNAscope probe Dm-Sand-C2	Advanced Cell Diagnostics	573821-C2
RNAscope probe Dm-AstA-C3	Advanced Cell Diagnostics	573861-C3
Opal dye 540	Akoya Bioscience	FP1494001KT
Opal dye 690	Akoya Bioscience	FP1497001KT
Opal dye 620	Akoya Bioscience	FP1495001KT
Opal dye Polaris 480	Akoya Bioscience	FP1500001KT
Deposited data
Experimental models: Cell lines
Experimental models: Organisms/strains
104y-Gal4	Bloomington Drosophila Stock Center	BDSC:81014; FlyBase: FBti0072312
23E10-Gal4	Bloomington Drosophila Stock Center	BDSC:49032; FlyBase: FBti0134066
UAS-smGFP-HA,LexAop-smGFP-V5	Bloomington Drosophila Stock Center	BDSC:64092; FlyBase: FBti0169244, FBti0169271
Sh RNAi	Vienna Drosophila Resource Center	VDRC: 104474
Hk RNAi	Vienna Drosophila Resource Center	VDRC: 101402
Sand RNAi	Vienna Drosophila Resource Center	VDRC: 47977
UAS-TrpA1	Seidner et al.35	Flybase: FBtp0040248
UAS-EKO	White et al.22	Flybase: FBtp0014962
UAS-Kir2.1	Baines et al.23	Flybase: FBto0000566
UAS-shits	Gerry Rubin	Flybase: FBtp0013545
tsh-Gal4	Julie Simpson	N/A
tsh-Gal80	Julie Simpson	N/A
Oligonucleotides
Recombinant DNA
Software and algorithms
NIS Elements Analysis AR 5.41.01	Nikon
ImageJ	Schneider et al.48	https://imagej.nih.gov/ij/
Prism 8	Graphpad
Other