Table 3.
Chemicals and reagents
| Chemicals and reagents | Source | Identifier |
|---|---|---|
| DMSO | Millipore Sigma | Cat# D2650-100ML |
| DAPT | Millipore Sigma | catalog # 565770-5MG |
| 10× HBSS | Thermo Fisher Scientific | catalog # 14–185-052 |
| 1 M Hepes | Thermo Fisher Scientific | catalog # 15–630-106 |
| 1 M D-Glucose | Thermo Fisher Scientific | Cat# J60067 |
| CaCl2 | Millipore Sigma | Cat# C4901 |
| MgSO4 | Millipore Sigma | Cat# M2643-500G |
| NaHCO3 | Millipore Sigma | Cat# S5761-500G |
| Basal Medium Eagle | Millipore Sigma | Cat# B1522-500ML |
| 200 mM L-glutamine | Gemini Bio | catalog # 400–106 |
| Penicillin-Streptomycin | Lonza Bioscience | Cat# DE17-602E |
| Millicell cell culture plate nserts | Millipore Sigma | Cat# Z353086 |
| Cell culture 6-well plates | Greiner Bio-one | catalog # 657160 |
| NEBuilder HiFi DNA Assembly Master Mix | New England Biolabs | Cat# E2621S |
| ProLong Diamond Antifade Mountant | Thermo Fisher Scientific | Cat# P36961 |
| Monarch MiniPrep Kit | New England Biolabs | Cat# T1010L |
| EndoFree Plasmid Maxi Kit | Qiagen | catalog # 12362 |
| Monarch DNA Gel Extraction Kit | New England Biolabs | Cat# T1020S |
| Monarch Genomic DNA Extraction Kit | New England Biolabs | Cat# T3010S |
| Monarch PCR & DNA Cleanup Kit | New England Biolabs | Cat# T1030L |
| Phusion High-Fidelity DNA Polymerase | New England Biolabs | Cat# M0530S |
| Neurobasal-A Medium (1×) | Gibco | catalog # 12349–015 |
| Papain Dissociation System | Worthington Biochem | Cat# LK003150 |
| MACS SmartStrainers (30 μm) | Miltenyi Biotec | catalog # 130–098-458 |