Figure 6.

IL-13 mRNA expression and secretion in the LPMCs of the small intestine in the indomethacin (IDM)-induced intestinal mucosal injury model. Twenty-four mice (12 mice each for the control and IDM groups) were used. The separated lamina propria mononuclear cells (LPMCs) were stimulated with PMA and ionomycin for 24 h. The LPMCs were counted (a). mRNA expressions of IL-13 in LPMCs were analyzed via real-time PCR, while IL-13 secretions in the culture supernatant of LPMCs were analyzed using ELISA (b,c). Statistical analyses were performed using the t test (a) and two-way analysis of variance (ANOVA) with Tukey’s post hoc test (b,c). ** p < 0.01, *** p < 0.001.