Table 3.

Antagonist activity of enantiopure β-lactam derivatives at rTRPM8 channels (Ca²⁺ microfluorimetry assays). Activation is induced by 100 µM menthol.

Compound	XR1	R2	R3	IC50 (µM)	95% Confidence Intervals
1	OtBu		Bn	1.06 ± 1.21	0.72 to 1.55
34	NHiBu		Bn	0.30 ± 1.26	0.19 to 0.50
35	NHBn		Bn	0.49 ± 1.27	0.30 to 0.79
36	NHCH2(2-Pic)		Bn	0.84 ± 1.32	0.48 to 1.49
37	OtBu		H	3.06 ± 1.25	2.67 to 3.51
40	OtBu		H	6.36 ± 1.41	3.08 to 13.17
41	OtBu		H	9.26 ± 1.41	4.44 to 19.35
42	NHiBu		H	2.19 ± 1.36	1.15 to 4.17
43	NHiBu		H	4.03 ± 1.35	2.14 to 7.60
44	NHiBu		H	3.27 ± 1.31	1.86 to 5.75
45	NHiBu		H	3.56 ± 1.40	1.74 to 7.31
AMTB				7.3 ± 1.50	6.82 to 7.85