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. 1998 May;42(5):1034–1041. doi: 10.1128/aac.42.5.1034

FIG. 3.

FIG. 3

Identification of three genes that confer resistance to QD when they are overexpressed in yeast. (A) CIN5 and STI1 are two genes that confer QD resistance and that are contained within the same QD-resistant primary transformant clone 2 (QDR7.3). The right plate depicts the plating scheme and the identification of transformants. The left plate shows equal growth of all yeast transformants in the absence of QD. The middle plate shows only growth of the yeast transformed with primary library clone 2 (QDR7.3) or with CIN5 and STI1 expressed under the control of the ADH promoter in pVT-U. (B) The ORF YOR273c contained in the QD-resistant primary transformant clone 3 (QDR9.13) confers QD resistance when it is overexpressed in yeast. The right plate depicts the plating scheme and the identities of the transformants. The left plate shows equal growth of all yeast transformants in the absence of QD. The middle plate shows only the growth of yeast transformed with primary library clone 3 (QDR9.3) and with YOR273c cloned in YEp24 or pVT-U (YOR273c) in the presence of QD.