Skip to main content
. Author manuscript; available in PMC: 2024 Oct 9.
Published in final edited form as: Dev Cell. 2023 Sep 25;58(19):1847–1863.e12. doi: 10.1016/j.devcel.2023.08.031

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
anti-GFP (goat) peroxidase conjugated Rockland 600-103-215
anti-RFP (Rabbit) Rockland 600-401-379
anti-HaloTag (Rabbit) Promega G9211
anti-Rabbit HRP-conjugated secondary antibody 1:3000 Cell signaling 7074S
monoclonal anti-beta-tubulin antibody Sigma T5201
Bacterial and virus strains
Escherichia coli: OP50 Strain Caenorhabditis Genetics Center (CGC) OP50
Escherichia coli: DH10B, chemical competent cells Thermo Fisher 18297010
Chemicals, peptides, and recombinant proteins
S. pyogenes Cas9 3NLS, 10μg/μl IDT Cat# 1081058
Auxin Alfa Aesar A10556
Casein Sigma C5890
Taxol (Paxitaxel) Cytoskeleton TXD01
AMP-PNP Sigma A2647
SuperSignal™ West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific 34579
SuperSignal™ West Femto Maximum Sensitivity Substrate Thermo Fisher Scientific 34094
GFP-trap agarose beads Chromotek gta
Critical commercial assays
EnGen sgRNA Synthesis Kit, S. pygenes NEB E3322S
Experimental models: Cell lines
HEK293T ATCC CB_12022001
COS7 ATCC CRL-1651
Experimental models: Organisms/strains
unc-70(syb1684[unc-70(1166R + AID + 1167D)]) (V) This study. phx1684
miro-1(wp119[7xGFP11::miro-1]) (IV), shyIs36 [Pmig-13::GFP1-10, Podr-1::RFP] (V) This study, wp119 has been generated by the Hammarlund lab, Youjun Wu, Yale School of Medicine, Departments for Neuroscience and Genetics MTS1291
oxTi185[ttTi5605 + NeoR(+) + unc-18(+)] (I), unc-119(ed3) (III) C. Frøkjær-Jensen, Jorgensen Lab, School of Biological Science, University of Utah EG8078
unc-44(ju1412[unc-44::GFP]) (IV) Fei Chen, Jin Lab, UCSD, School of Biological Sciences, ordered through CGC CZ24990
spc-1(cas1047[spc-1::7×GFP11]) (X); wpIs141[Psra-6::GFP1-10, Pmyo-2::mCherry] (II) This study MTS742
spc-1(cas1047[spc-1::7×GFP11]) (X); shyEx155[Pmig-13::GFP1-10, Podr-1::RFP] This study MTS743
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS826
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V) This study MTS879
spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS1082
spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V) This study MTS1067
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V); shyEx305[Pmig-13::TIR1, Podr-1::RFP]; unc-70(syb1684[unc-70(1166R + AID + 1167D)]) (V) This study MTS1006
unc-70(shy112 [7xGFP11::unc-70]) (V); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V) This study MTS1510
unc-70(shy112 [7xGFP11::unc-70]) (V); shyIs36 (V); spc-1(shy111[spc-1::aid]) (X); shyIs53[Pmig-13::TIR1, Podr-1::RFP] (II) This study MTS1515
unc-116(e2310) (III); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS953
unc-116(e2310) (III); shyEx369[Pmig-13::unc-116::SL2::mCherry, Podr-1::GFP]; spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS1360
unc-116 (shy92 [unc-116::AID]) (III); shyEx305[Pmig-13::TIR1, Podr-1::RFP]; spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS1489
unc-116(rh24sb79) (III); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 (IV) This study MTS954
unc-76(shy19[zf-1::unc-76]) (V); shyIS16 [Pmig-13::ZF-1, Pttx-3::RFP] (V); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS951
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV); shyEx78[Pmig-13::FEZ1, Pmig-13::STOP::RFP::Podr-1::GFP] ; unc-76 (shy19[zf-1::unc-76]) (V); shyIs16[Pmig-13::ZF-1, Pttx-3::RFP] (V) This study MTS1003
unc-69(syb2774[unc-69::zf-1::7xGFP11])(III); shyIs9[Pmig-13::ZF-1, Pttx-3::RFP] (IV); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP](V) This study MTS1337
unc-69(syb2774[unc-69::zf-1::7xGFP11])(III); shyIs9 [Pmig-13::ZF-1, Pttx-3::RFP] (IV); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP](V); shyEx381[Pmig-13::SCOC, Podr-1::GFP] This study MTS1433
spc-1(cas1047[spc-1::7×GFP11]) (X), shyEx155[Pmig-13::GFP1-10, Podr-1::RFP]; unc-76(shy19[zf-1::unc-76]) (V); shyIs16[Pmig-13::ZF-1, Pttx-3::RFP] (V);unc-69(syb2774[unc-69::zf-1::7xGFP11])(III) This study MTS877
unc-116(e2310) (III); unc-76(shy19[zf-1::unc-76]) (V);shyIs16[Pmig-13::ZF-1, Pttx-3::RFP] (V); spc-1(cas1047[spc-1::7×GFP11]) (X); shyEx155[Pmig-13::GFP1-10, Podr-1::RFP] This study MTS813
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV); jip-1(gk133506) (II) This study MTS1484
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV); unc-16(ce483) (III) This study MTS1290
klc-2(km11), klc-1(ok2609), spc-1(cas1047[spc-1::7×GFP11]) (X), shyEx155[Pmig-13::GFP1-10, Podr-1::RFP] This study MTS827
unc-104(e1265); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV) This study MTS906
spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs35 [Pmig-13::GFP1-10, Podr-1::RFP] (IV); unc-76(shy19[zf-1::unc-76])(V); shyIs16[Pmig-13::ZF-1, Pttx-3::RFP] (V) This study MTS1167
spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV), unc-116(e2310) (III) This study MTS1168
unc-116(e2310); unc-70(shy112 [7xGFP11::unc-70]) (V); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V) This study MTS1516
unc-69(syb2774[zf-1::7xGFP11::unc-69])(III); shyIs9[Pmig-13::ZIF-1, Pttx-3::RFP] (IV); unc-70(shy112 [7xGFP11::unc-70]) (V), shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V) This study MTS1535
unc-44 (ju1412[unc-44::GFP]) (IV); shyEx415[Prab-3::RFP, Punc-122::RFP] This study MTS1688
unc-44 (ju1412[unc-44::GFP]) (IV); shyEx415[Prab-3::RFP, Punc-122::RFP]; unc-116(e2310) (III) This study MTS1689
unc-44 (ju1412[unc-44::GFP]) (IV); shyEx415[Prab-3::RFP, Punc-122::RFP]; unc-76(e911) (V) This study MTS1693
spc-1(cas1047[spc-1::7×GFP11]) (X); wpIs141[Psra-6::GFP1-10, Pmyo-2::mCherry] (II); unc-76(e911)(V); shyEx396[Psra-6::tagRFP, Podr-1::GFP] This study MTS1462
spc-1(cas1047[spc-1::7×GFP11]) (X); wpIs141[Psra-6::GFP1-10, Pmyo-2::mCherry] (II); unc-116(e2310) (III) This study MTS1517
shyEx268[Pmig-13::GFP::RAB11.1, Podr-1::RFP] This study MTS1049
unc-76 (shy19[zf-1::unc-76])(V); shyIs16 [Pmig-13::ZF-1, Pttx-3::RFP] (V); shyEx268[Pmig-13::GFP::RAB11.1, Podr-1::RFP] This study MTS1197
unc-116(e2310); shyEx268[Pmig-13::GFP::RAB11.1, Podr-1::RFP] This study MTS1306
tba-1(ok1135) (I); wySi256/+ [Punc-4C::CRE, Punc-122::RFP] (II); him-8 (IV); wyIs463[Pmig-13::STOP::GFP::TBA-1], shyIs16 [Pmig-13::ZF-1, Pttx-3::RFP] (V), unc-76(shy19[zf-1::unc-76])(V); wyEx2709 This study MTS1303
unc-76(shy45[10xGFP11::unc-76]) (V); shyEx155 [Pmig-13::GFP1-10, Podr-1::RFP] This study MTS598
unc-116(e2310) (III); unc-76(shy45[10xGFP11::unc-76]) (V); shyEx155 [Pmig-13::GFP1-10, Podr-1::RFP] This study MTS652
unc-69(syb2774[zf-1::7xGFP11::unc-69])(III); unc-76(shy45[10xGFP11::unc-76]) (V); shyEx155 [Pmig-13::GFP1-10, Podr-1::RFP] This study MTS1071
unc-69(syb2774[zf-1::7xGFP11::unc-69]) (III) ; shyEx155 [Pmig-13::GFP1-10, Podr-1::RFP] This study MTS839
unc-69(syb2774[zf-1::7xGFP11::unc-69])(III); unc-76(e911) (V); shyEx155 [Pmig-13::GFP1-10, Podr-1::RFP] This study MTS1072
unc-76(shy19[zf-1::unc-76]) (V); shyIS16[Pmig-13::ZF-1, Pttx-3::RFP] (V); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV); shyEx370[Pmig-13::KLC-2(IsoformA)::tagRFP::vhhGFP4, Podr-1::GFP] This study MTS1361
unc-76(shy19[zf-1::unc-76]) (V); shyIS16[Pmig-13::ZF-1, Pttx-3::RFP] (V); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV); shyEx380[Pmig-13::KLC-2(IsoformA)::tagRFP, Podr-1::GFP] This study MTS1420
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV); shyEx336[Pmig-13::tagRFP::UNC-76(IsoformA), Podr-1::GFP] This study MTS1256
shyEx336[Pmig-13::tagRFP::UNC-76(IsoformA), Podr-1::GFP]; miro-1(wp119[7xGFP11::miro-1) (IV); shyIS36 (V) This study MTS1413
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (IV); shyEx336[Pmig-13::tagRFP::UNC-76(IsoformA), Podr-1::GFP]; unc-116(e2310) (III) This study MTS1665
unc-69(syb2774[zf-1::7xGFP11::unc-69]) (III); shyIs9[Pmig-13::ZF-1, Pttx-3::RFP] (IV); spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP](V); shyEx336[Pmig-13::tagRFP::UNC-76(IsoformA), Podr-1::GFP] This study MTS1710
dhc-1(shy179(H2159L)) (I); spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V) This study MTS2148
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs40[Pitr-1::GFP1-10, Podr-1::RFP] (I) This study MTS1720
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs40[Pitr-1::GFP1-10, Podr-1::RFP] (I), unc-115(ky275) (X) This study MTS1729
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs40[Pitr-1::GFP1-10, Podr-1::RFP] (I), unc-115(shy159[M1STOP]) (X) This study MTS2212
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (I), unc-115(ky275) (X) This study MTS2250
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs35[Pmig-13::GFP1-10, Podr-1::RFP] (I), unc-115(ky275) (X); unc-76 (shy19[zf-1::unc-76])(V); shyIs16 [Pmig-13::ZF-1, Pttx-3::RFP] (V) This study MTS2249
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs83[Pmec-17::GFP1-10] (I) This study MTS2000
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs83[Pmec-17::GFP1-10] (I); unc-115(ky275) (X) This study MTS2251
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs83[Pmec-17::GFP1-10] (I); unc-70(syb1684[unc-70(1166R + AID + 1167D)]) (V); shyEx517[Pmec-17::tir1] This study MTS2093
spc-1(cas1047[spc-1::7×GFP11]) (X); shyIs83[Pmec-17::GFP1-10] (I); unc-76 (shy19[zf-1::unc-76])(V); shyEx536[Pmec-17::ZIF-1] This study MTS2154
unc-115(shy161[3xGFP11::unc-115]) (X); shyIs83[Pmec-17::GFP1-10] (I) This study MTS2215
spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V); shyEx540 [Pmig-13::UNC-76] This study MTS2226
spc-1 (shy84 [spc-1::FRT::let-858UTR::FRT::7xgfp11]) (X); shySi4 [(hsp16.4p::FLP, unc-119(+))*oxTi185] (I); shyIs36[Pmig-13::GFP1-10, Podr-1::RFP] (V); unc-76 (shy19[zf-1::unc-76])(V); shyIs16[Pmig-13::ZF-1, Pttx-3::RFP] (V); shyEx548[Pmig-13::KLC-2::vhhGFP4] This study MTS2290
Oligonucleotides
Oligonucleotides were ordered from SigmaAldrich. A list of all oligonucleotides used in this study is provided in supplemental table S2. Sigma Aldrich See table S2.
Single stranded oligonucleotides that were used as CRISPR repair templates were ordered from IDT and are also listed in table S3. IDT See table S3.
Recombinant DNA
pRF4::rol-6(su1006) Melo CC, Ambros Lab, UMass, Program in Molecular Medicine pRF-4
Prab-3::mCherry::unc-54utr C. Frøkjær-Jensen, Jorgensen Lab, School of Biological Science, University of Utah pGH8
Phsp-16.4::FLP::let-858utr_pXF87 backbone This study pOVG19
Phsp-16.41:peel-1:tbb-2utr C. Frøkjær-Jensen, Jorgensen Lab, School of Biological Science, University of Utah pMA122
Pmyo-2::mCherry::unc-54utr C. Frøkjær-Jensen, Jorgensen Lab, School of Biological Science, University of Utah pCFJ90
Pmig-13::UNC-116::SL2::mCherry This study pOVG26
Pmig-13::tagRFP::UNC-76 This study pOVG36
Pmig-13::KLC-2(isoformA)::tagRFP This study pOVG43
Pmig-13::KLC-2(isoformA)::tagRFP::vhhGFP4 This study pOVG44
Pmig-13::SCOC This study pMR18
Pmig-13::FEZ1 This study pMR20
Pmig-13::KLC-2(isoformA)::vhhGFP4 This study pOVG63
Pmec-17::zif1 This study pOVG67
Pmec-17::3xGFP This study pOVG70
Pmec-17::tir1 This study pOVG65
Pmec-17::GFP1–10 This study pOVG56
Pmig-13::UNC-76 This study pMR13
Pcmv::SPTBN1(Q01082-1)::eGFP This study pOVG40
Pcmv::HALO::SCOC This study pGLS5
Pcmv::mCherry::FEZ1 This study pOVG46
Pcmv::SNAP::FEZ1 This study pGLS25
Software and algorithms
Prism Version 7 GraphPad
Illustrator Adobe
RStudio R
Image J NIH
EndNote20 EndNote
Algorithm: Spectrin-Axonal-Mobility-Model. Generated by: Gramlich lab, Michael Gramlich, Auburn University, Physics department
DOI: https://zenodo.org/badge/latestdoi/615469432 		Code is available on github: https://github.com/AU-Neurophysics-Lab/Spectrin-Axonal-Mobility-Model.