Table 4.
Anti-inflammatory activity of H. sanjappae extract against lipopolysaccharide-induced activation of Raw 264.7 cells and comparison with standard aspirin (1 mM).
| IL-1β (pg/mg Protein) |
IL-6 (pg/mg Protein) |
TNF-α (pg/mg Protein) |
NO (µM/mg Protein) |
|
|---|---|---|---|---|
| Untreated | 64.6 ± 1.9 | 133.4 ± 5.8 | 115.2 ± 3.1 | 10.7 ± 0.64 |
| LPS Control | 573.4 ± 4.5 | 628.5 ± 8.2 | 856.0 ± 11.2 | 75.2 ± 2.1 |
| Aspirin (1 mM) | 147.5 ± 5.1 *** | 209.5 ± 9.1 *** | 247.5 ± 6.3 *** | 22.7 ± 1.7 *** |
| HSE 5 µg/mL | 403.7 ± 6.2 ** | 507.1 ± 8.1 * | 715.2 ± 8.8 * | 58.8 ± 3.4 * |
| HSE 10 µg/mL | 298.5 ± 8.4 *** | 388.4 ± 2.8 *** | 602.8 ± 5.2 *** | 42.3 ± 1.9 *** |
| HSE 20 µg/mL | 156.2 ± 3.4 *** | 291.3 ± 6.6 *** | 493.7 ± 6.4 *** | 30.7 ± 2.5 *** |
* indicates significant variation with respect to LPS control (p < 0.05); ** indicates higher significant variation with respect to that of LPS control (p < 0.01), and *** indicates highest significant variation with respect to that of LPS control (p < 0.001). All the results are indicated as mean ± standard deviation of six independent experiments.