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The investigated independent factors at 3 levels in the optimization process of PIO-loaded polymeric micelles.
| Levels | |||
|---|---|---|---|
| Independent Factors | −1 | 0 | +1 |
| SA concentration (mg/mL) | 4.0 | 5.0 | 6.0 |
| Ca2+ concentration (mg/mL) | 3.0 | 6.0 | 9.0 |
| Flow rate (ml/min) | 1.0 | 2.0 | 3.0 |
