Figure 2.

SDS-induced aggregation of lysozyme monitored by ThT fluorescence and turbidity: effect of acetone and controls. (A) Reaction kinetics monitored by fluorescence and (B) fluorescence spectra. Reaction conditions: lysozyme (10 µM), ThT (10 µM), SDS (250 µM) in 50 mM phosphate buffer, pH 7.0. The reactions were conducted in the absence and presence of acetone (AC, 10% v/v). (C) Reaction kinetics monitored by turbidity. The reaction conditions were the same except for the absence of ThT. (D) TEM micrography of lysozyme amyloid fibrils produced by SDS (250 µM) in the presence of acetone (10%). The image was viewed at 12,000x magnification.