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Lag phase induced by acetone concentration on SDS-induced aggregation of lysozyme and effect of temperature: (A) Time-dependent profile as a function of acetone concentration. (B) Effect of temperature at fixed acetone concentration (10%). Reaction conditions: lysozyme (10 µM), ThT (10 µM), SDS (250 µM) in 50 mM phosphate buffer, pH 7.0.
