Fig. 5. CEPsh glia require functional UNC-13 (SCV), while neurons require UNC-31 (DCV), to activate UPR^MT in the periphery.

(A) Schematic of spatial mutation strategy. Expression of the flipase FLP D5 under the CEPsh glial-specific (hlh-17p) or pan-neuronal (rgef-1p) promoters, in combination with an independent FRT (FLP recognition target) allele of a gene of interest results in a tissue-specific mutation. (B) Representative fluorescent micrographs of the indicated strains at day 1 adult animals. Scale bar, 250 μm. (C and D) Median spatial profiles of the indicated animals (see Methods), for depletion in CEPsh glial cells (C) or neuronal cells (D) quantified with large particle biosorter (n > 50). The integrated fluorescence intensity of the 30% most posterior portion of the animals was calculated and plotted in (E). One-way ANOVA with Tukey’s multiple comparisons test, ***P < 0.001 and ****P < 0.0001.