|
Experimental models
|
| N2 Bristol |
Caenorhabditis Genetics Center, University of Minnesota, USA |
N/A |
| VC299 |
Caenorhabditis Genetics Center, University of Minnesota, USA |
N/A |
| NM4325 |
Gift of Professor Michael Nonet, Washington University Medical School, USA |
N/A |
| NM4031 |
Gift of Professor Michael Nonet, Washington University Medical School, USA |
N/A |
| RW1596 |
Gift of Professor Bart Braeckman, Ghent University, Belgium |
N/A |
| PHX7030 |
SunyBiotech, China |
N/A |
| LSC1898 |
This study |
N/A |
| LSC1950 |
N/A |
| LSC1951 |
N/A |
| LSC1959 |
N/A |
| LSC1870 |
N/A |
| LSC1960 |
N/A |
| LSC1998 |
N/A |
| LSC1999 |
N/A |
| LSC2000 |
N/A |
| LSC2001 |
N/A |
| LSC2052 |
N/A |
| LSC2053 |
N/A |
| LSC2055 |
N/A |
|
Recombinant DNA
|
|
azyx-1p::azyx-1::mNeonGreen::UTR |
This study |
N/A |
|
azyx-1p::azyx-1(gBlock)::UTR
|
This study |
N/A |
|
unc47p::mCherry
|
This study |
N/A |
|
unc133p::mCherry
|
This study |
N/A |
|
myo2p::mCherry
|
This study |
N/A |
|
Oligonucleotides and sequence-based reagents
|
This study |
S2 Table
|
|
Chemicals, enzymes and other reagents
|
| Spike-in peptide EAVSEILETSR |
GL Biochem |
N/A |
| Modified Trypsin |
Promega |
V5111 |
| Formic Acid |
Pierce |
85178 |
| Acetonitrile |
Sigma/Merck |
100029 |
| Dimethyl sulfoxide |
Sigma |
276855 |
| Pluronic F-127 |
Sigma |
P2443 |
| SspI Fast Digest |
Thermo Scientific |
FD0774 |
| NEBuilder HiFi DNA assembly |
New England Biolabs |
E2621 |
| Fluorodeoxyuridine |
Sigma |
F0503 |
| Dithiothreitol |
Serva |
20710 |
| Iodoacetamide |
Serva |
26710 |
| Urea |
Sigma |
U5378 |
| Thiourea |
Sigma |
88810 |
| HEPES |
Sigma |
H3375 |
|
Software
|
| MaxQuant (v1.6.17.0) with Andromeda |
[53] |
N/A |
| Skyline (version 64-bit 21.1.0.278, 22.2.0.527) |
[54] |
N/A |
| Prosit |
[55] |
N/A |
| MSStats |
[56] |
N/A |
| Fiji |
https://imagej.net/software/fiji
|
N/A |
| Blinder |
[57] |
N/A |
| Rstudio |
https://www.rstudio.com/
|
N/A |
| Inkscape |
https://inkscape.org
|
N/A |
| Affinity Designer |
https://affinity.serif.com
|
N/A |
|
Other
|
| iRT Kit |
Biognosys |
N/A |
| Wizard Genomic DNA purification kit |
Promega |
A1120 |
| COPAS |
Union Biometrica |
|
| Pierce C18 spin columns |
Thermo Scientific |
89870 |
| UltiMate 3000 RSLCnano liquid chromatography system |
Thermo Fisher Scientific |
N/A |
| Q-Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer |
Thermo Fisher Scientific |
N/A |
| sssReproSil Gold C18-AQ, 3 μm, 450 mm × 75 μm |
Dr. Maisch Gmbh |
r13.aq.0003 |
| ReproSil-pur C18-AQ, 5 μm, 20 mm × 75 μm |
Dr. Maisch Gmbh |
r15.aq.0001 |
