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. Author manuscript; available in PMC: 2024 Nov 1.
Published in final edited form as: FASEB J. 2023 Nov;37(11):e23251. doi: 10.1096/fj.202301122RR

Figure 4. Whole body silencing Lpcat3 expression has no effect on lipid and energy metabolism in HFD-fed mice.

Figure 4.

A-D. Serum triglyceride (A) and NEFA (B) levels and liver triglyceride (C) and NEFA (D) levels of HFD-fed C57BL/6 mice injected with Con-ASO or Lpcat3-ASO.

E. Expression of selected genes in the livers of HFD-fed C57BL/6 mice injected with Con-ASO or Lpcat3-ASO (n=12/group).

F. Postprandial lipid absorption analysis in HFD-fed C57BL/6 mice injected with Con-ASO or Lpcat3-ASO (n=4–5/group).

G. Representative histology of liver, eWAT and sWAT from HFD-fed C57BL/6 mice injected with Con-ASO or Lpcat3-ASO. Scale bar: 100 μm.

H-I. Energy expenditure (EE) analyzed by regression analysis of covariance (ANCOVA).

J. EE estimated by univariate generalized linear model (GLM) with body mass set to 39.14 g (average body mass of Con-ASO or Lpcat3-ASO injected mice on HFD).

K-L. Physical activity (K) and respiratory exchange ratio (RER) (L) in HFD-fed Lpcat3fl/fl mice injected with Con-ASO or Lpcat3-ASO.

Data are presented as means ± SEM. Statistical analysis was performed with Student’s t test (A-E), ANCOVA (H-I), and two-way ANOVA (F, J-L). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.