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. 2023 Oct 16;133(20):e169671. doi: 10.1172/JCI169671

Figure 1. Antioxidants stabilize BACH1 and induce angiogenesis gene expression in NSCLC organoids and tumors by upregulating BACH1 expression.

Figure 1

(A) Experimental design. (B) BACH1 protein levels in spheroids incubated for 7 days with 25 μM VitC and BACH1 levels by densitometry (n = 3 experiments). (C) Reverse transcription quantitative PCR (RT-qPCR) of BACH1 in A549 and H838 spheroids , lung tumor organoids incubated for 7 days with 25 μM VitC, and A549 xenograft tumors from mice administered VitC (3.47 g/L) in the drinking water for 7 weeks (n = 3 experiments). (D) RT-qPCR of angiogenesis genes in spheroids incubated with 25 μM VitC for 7 days (n = 3 experiments). (E) VEGFR2 protein levels in spheroids incubated with 25 μM VitC for 7 days and VEGFR2 levels determined by densitometry (n = 6 experiments). Ctrl, control. Data indicate the mean ± SEM. Statistical significance was determined by 2-tailed, unpaired Student’s t test (C–E) and 1-way ANOVA with Tukey’s post hoc test for multiple comparisons (B).