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. 2023 Oct 16;133(20):e169671. doi: 10.1172/JCI169671

Figure 2. BACH1 controls the expression of angiogenesis genes under normoxia.

Figure 2

(A) RT-qPCR of angiogenesis genes in BACH1OE and BACH1WT spheroids under normoxia (n = 3 experiments). (B) VEGFR2 protein levels in BACH1OE and BACH1WT spheroids and VEGFR2 levels by densitometry (n = 4 experiments). (C) RT-qPCR of angiogenesis genes in BACH1+/+ and BACH1–/– spheroids under normoxia (n = 4 experiments). (D) VEGFR2 protein levels in BACH1+/+ and BACH1–/– spheroids and VEGFR2 levels by densitometry (n = 3 experiments). (E) RT-qPCR of angiogenesis genes in BACH1+/+ and BACH1–/– spheroids incubated for 7 days with 25 μM VitC or vehicle (Ctrl) (n = 3 experiments). (F) Top, VEGFR2 and BACH1 protein levels in BACH1+/+ and BACH1–/– spheroids incubated for 7 days with 25 μM VitC and VEGFR2 protein levels by densitometry (n = 4 experiments). Data indicate the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.005, and ****P < 0.001, by 2-tailed, unpaired Student’s t test (A–D) and 1-way ANOVA with Tukey’s post hoc test for multiple comparisons (E and F).