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. 2023 Oct 16;133(20):e170173. doi: 10.1172/JCI170173

Figure 2. Knockout of PCIF1 suppresses the HNSCC malignant phenotype.

Figure 2

(A) Western blotting analyses of the PCIF1 expression in the cell lines. (B) Western blotting analyses detecting the PCIF1 expression in SCC9 (left) and SCC25 (right) control cells and PCIF1-KO cells. (C) Cell Counting Kit-8 (CCK8) assay of cell viability in control and PCIF1-KO cells (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 by 1-way ANOVA, Dunnett’s test. (D) Colony formation assay detecting the colony ability of control and PCIF1-KO cells (n = 3). ***P < 0.001 by 1-way ANOVA with Tukey’s multiple-comparison test. (E and F) The cell migration (E) and invasion (F) ability of control and PCIF1-KO cells was determined by Transwell assay (n = 3). **P < 0.01, ***P < 0.001 by 1-way ANOVA with Tukey’s multiple-comparison test. Scale bar: 100 μm. (G) Flow cytometry assay for cell apoptosis in control and PCIF1-KO cells. Bottom: Representative images. Top: Quantification data (n = 3). ***P < 0.001 by 1-way ANOVA with Tukey’s multiple-comparison test. (H) Cell cycle progression was detected by flow cytometric analyses in control and PCIF1-KO cells (n = 3). **P < 0.01, ***P < 0.001 by 1-way ANOVA with Tukey’s multiple-comparison test.