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. 2023 Oct 16;133(20):e161366. doi: 10.1172/JCI161366

Figure 7. VEGF-induced PKC signaling mediates eNOS activation and junctional tyrosine nitration of Src.

Figure 7

(A) Western blot showing VEGFA-activated signaling in HUVECs pretreated with DMSO (control) or the PKC inhibitor Go6983. (B–E) Quantification of at least 3 independent experiments from A, for peNOS S1177 (B), pSFK Y418 (C), pERK (D), and pPLCγ Y783 (E), shown as fold change of unstimulated samples. 1-way ANOVA. (F) PLA using antibodies against 3-nitrotyrosine and pSFK Y418, in HUVECs stimulated with VEGFA (100 ng/mL, 5 minutes), pretreated with siCtr or siNOS3. Junctions are stained for VE-cadherin (VEC). Scale bar: 20 μm. Boxed regions in left panels are magnified in panels to the right. Scale bar: 5 μm. (G) MFI quantification of junctional 3-nitrotyrosine/pSFK Y418 PLA signals from F; n = 3 independent experiments, ≥ 3 fields of view/experiment. 1-way ANOVA. Data represent mean ± SD. *P < 0.05, ***P < 0.001.