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. 2023 Jul 10;18(10):1241–1251. doi: 10.1038/s41565-023-01419-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, CAP-Mac after intracerebroventricular (ICV) administration in adult mice primarily transduces neurons, mimicking the CAP-Mac tropism in infant Old World primates after intravenous (IV) administration. b, CAP-Mac after IV administration in C57BL/6 J, BALB/cJ, and DBA/2 J adult mice primarily transduces vasculature, with no apparent differences between the three mouse strains. c, CAP-Mac in P0 C57BL/6 J pups after intravenous administration transduces various cell-types, including neurons, astrocytes, and vasculature. d-g, Given the neuronal tropism of CAP-Mac via ICV administration, we validated GCaMP cargo in mice prior to non-human primate experiments, testing either one-component or two-component systems. d, e, GCaMP protein expression and representative ΔF/F traces in mice after delivering CAG-GCaMP6f (n = 2 mice) (d) or a CAG-tTA/TRE-GCaMP6f cocktail (n = 3 mice) using CAP-Mac (e). f, g, To determine cargo to move forward with, we found that 0-3 Hz bandpower (two-tailed Welch’s t-test, P = 0.105) (f), but not area under the curve (AUC; two-tailed Welch’s t-test, P = 0.626) (g), was indicative of cargo performance.