Figure S6. Further correlated NanoSIMS and fluorescent images from plastic-embedded samples labeled with synaptotagmin-1 (Syt1), an anti-mouse nanobody (NB), and synaptophysin (Syph).

⁴⁰Ca images (leftmost column) were generated using NanoSIMS, which was performed after fluorescence analysis. Brighter colors indicate higher ⁴⁰Ca intensity. Arrowheads in the overlay figure point to artefacts linked to the strong autofluorescence of the nuclei in the plastic-embedded samples. Such regions were not analyzed. Scale bar: 10 μm.