FIG. 2.

CNI-H0294 inhibits 2-LTR circle formation in infected cells. Anti-CD3 MAb-activated PBMC cultures (a) or Jurkat cell cultures (b) were preincubated with CNI-H0294 and were infected with either HIV-1_M1 (20 ng of p24/10⁶ cells) for 40 h (a) or HIV-1_LAI (50 ng of p24/10⁶ cells) for 20 h (b) in the presence of the compound. The presence of 2-LTR circle forms of the HIV-1 DNA was a measure of nuclear translocation, while PCR analysis of gag sequences was used as a measure of the overall HIV-1 DNA content, reflecting viral entry into the cells and reverse transcription.