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. 2023 Oct 14;6:1043. doi: 10.1038/s42003-023-05407-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Global lipidomic profiling of plasma (PL), bone marrow (BM), and bone powder (BP) samples obtained from investigated mice using LC-MS. Heatmap of the sum of the abundances of unique lipid species for each lipid class for (a) plasma, (b) bone marrow, and (c) bone powder with group averages (n = 5-10 per group). Lipid classes statistically altered are marked by an asterisk (*) based on ANOVA with p(FDR) < 0.05. Differences between groups are marked as 1 (ND vs. HFD; p < 0.05), 2 (ND vs. HFD + F; p < 0.05), and 3 (HFD vs. HFD + F; p < 0.05) (gradient color keys used in normalized intensity, A.U.). (Lipid class annotation: CAR acylcarnitine, CE cholesteryl ester, CL cardiolipin, Ce, ceramide, CoQ coenzyme Q, DG diacylglycerol, EtherPE ether-linked phosphatidylethanolamine, EtherLPE ether-linked lysophosphatidylethanolamine, EtherPI ether-linked phosphatidylinositol, EtherPC ether-linked phosphatidylcholine, EtherTG ether-linked triacylglycerol, FA free fatty acid, FAHFA fatty acid ester of hydroxy fatty acid, HexCer hexosylceramide, LPC lysophophatidylcholine, LPE lysophosphatidylethanolamine, LPG lysophosphatidylglycero, LPI lysophosphatidylinositol, LPS lysophosphatidylserine, MG monoacylglycerol, NAE N-acyl ethanolamines, OxPE oxidized phosphatidylethanolamine, PC phosphatidylcholine, PE phosphatidylethanolamine, PEtOH phosphatidylethanol, PG phosphatidylglycerol, PI phosphatidylinositol, PS phosphatidylserine, SM sphingomyelin, SSulfate sterol sulfate, TG triacylglycerol).