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. 2023 Oct 14;6:1043. doi: 10.1038/s42003-023-05407-8

Fig. 5. Omega-3 PUFAs affect the differentiation capacity of OCs in obese mice.

Fig. 5

a–c Analysis of osteoclast differentiation from BM cells isolated from treated mice. a Number of TRAP+ cells, (b) Proportion of large TRAP+ OCs from total OCs (cell diameter above 500 µm) and (c) measurement of TRAcP activity normalized to number of TRAP+ cells (n = 5–6 per group; one-way ANOVA, Tukey’s multiple comparison test with *p ≤ 0.05) Data are presented as mean ± SEM. d Representative pictures of TRAP+ cells after 5 days of differentiation. Gene expression analysis of (e) oscteoclastic genes (Trap, Ctsk) and (f) inflammatory genes (Il1β, Tnfα, Rela) measured in OC differentiated cells after 5 days of differentiation. (n = 5–6 mice per group; one-way ANOVA, Tukey’s multiple comparison test with * p ≤ 0.05). Data are presented as mean fold change (F.C.) of gene expression normalized to ND group ± SEM (groups coding: white column-ND, black line shading-HFD, yellow column-HFD + F).