Fig. 1. NPC1L1 endocytosis is essential for cholesterol absorption.

a The schematic model of Npc1l1-Y₁₃₀₆VNYGF → AAAYGA (4A) knock-in mouse. +/+: Wild-type (WT); UTR: Untranslated region. b Filipin staining of intestinal sections from WT male mice (+/+) or 4A knock-in male mice (4A/4A) receiving an oral gavage of 30 mg/mL cholesterol in 100 μL corn oil (+ Cholesterol group) or 100 μL corn oil (- Cholesterol group). Scale bar, 10 μm (main); 1 μm (inset). The relative level of ³H-cholesterol in the liver (c) and plasma (d) in the WT (+/+), heterozygous (+/4A) or homozygous (4A/4A) 4A knock-in male mice. Chol, cholesterol. Values were presented as mean ± SD (n = 4 mice). One-way ANOVA with Tukey post hoc test, *P < 0.5; **P < 0.01. Source data are provided as a Source Data file.