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. 2023 Oct 13;14:6469. doi: 10.1038/s41467-023-42179-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a A 10-min treatment of vehicle, BA mixture or CDX in CRL1601/NPC1L1-3×Myc-EGFP cells transfected with pCMV-LIMA1-mCherry. Scale bar, 10 μm. The relative intensity of LIMA1-mCherry (b) and cholesterol (c) resided in ERC in (a) was quantified. Values were presented as mean ± SD (n = 3 independent trials, 100 cells/trial). Two-way ANOVA with Tukey post hoc test, ***P < 0.001. ns, no significance. d Control or Ntcp knockdown CRL1601/NPC1L1-3×Myc-EGFP cells with a 10-min incubation of vehicle, indicated BAs. Scale bar, 10 μm. The relative intensity of cholesterol (e) and LIMA1-mCherry (f) resided in ERC in (d) was quantified. Values were presented as mean ± SD (n = 3 independent trials, 100 cells/trial). Two-way ANOVA with Tukey post hoc test, ***P < 0.001. ns, no significance. g CRL1601/NPC1L1-3×Myc-EGFP cells were transfected with pCMV-LIMA1-FLAG for 48 h and subjected to the co-immunoprecipitation by the anti-FLAG agarose after a 10-min treatment of indicated BAs or 3% CDX (n = 2). Chol., Cholesterol; Veh, Vehicle; Mix., Mixture. Source data are provided as a Source Data file.