Product name | Catalog number | Company |
---|---|---|
Chemicals | ||
Sangivamycin | HY-118384 | MedChemExpress |
Rapamycin | HY-10219 | MedChemExpress |
Torin-1 | HY-13003 | MedChemExpress |
Antibodies | ||
Rabbit anti-ATF3 (WB) | #33593 | Cell Signaling |
Rabbit anti-ATF4 | #118115 | Cell Signaling |
Rabbit anti-TRIB3 | #43043 | Cell Signaling |
Rabbit anti-DDIT4 | #10638-1-AP | Proteintech |
Mouse anti-actin | #sc-47778 | Santa Cruz Biotechnology |
Mouse anti-tubulin | #sc-5286 | Santa Cruz Biotechnology |
Rabbit anti-ATF3 (IP) | #18665 | Cell Signaling |
Secondary anti-Rabbit | #31430 | Thermo Scientific |
Secondary anti-Mouse | #31460 | Thermo Scientific |
Ki67(IHC) | #M7240 | Agilent Technologies |
p27 | #M7203 | Agilent Technologies |
ATF3 | #MA5-31360 | Thermo Scientific |
Anti-Mouse IgG Reagent | #MP-7402 | Vector Laboratories |
DAB Substrate Kit | #SK-4100 | Vector Laboratories |
Cell lines | ||
HCT116 | #CCL-247 | ATCC |
DLD-1 | #CCL-221 | ATCC |
HT-29 | #HTB-38 | ATCC |
HEK293T | #CRL-3216 | ATCC |
Reagents and consumables | ||
McCoy’s 5A | #16600082 | Thermo Scientific |
DMEM medium | #10569010 | Thermo Scientific |
Fetal Bovine Serum | #10270106 | Thermo Scientific |
Penicillin-Streptomycin | #15140-122 | Thermo Scientific |
Resazurin powder | #R7017 | Sigma-Aldrich |
Antigen unmasking solution | #H-3300 | Vector Laboratories |
2.5% Normal Horse Serum | #S-2012 | Vector Laboratories |
Hematoxylin | #S3309 | Agilent Technologies |
Mounting buffer | #S3025 | Agilent Technologies |
Tris-Cl PH 8.0 | #15568-025 | Invitrogen |
EDTA | #AM9290G | Invitrogen |
1% SDS | #15553-035 | Invitrogen |
0.2 M NaCl | #AM9759 | Invitrogen |
Proteinase K | #EO0491 | Thermo Scientific |
Histogel | #HG-4000-012 | Fisher Scientific |
96-well ultra-low attachment plates | #7007 | Corning |
RIPA lysis buffer | #89901 | Fisher Scientific |
4–12% SDS-PAGE | #NP0336BOX | Thermo Scientific |
MinElute PCR Purification kit | #28004 | QIAGEN |
Dynabeads™ Co-Immunoprecipitation Kit | #14321D | Thermo Scientific |