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. 2023 Oct 13;9:375. doi: 10.1038/s41420-023-01672-y

Fig. 2. Combination of PL with 17-AAG increased ROS production and induced DNA damage.

Fig. 2

A, B Intracellular ROS levels in RKO cells treated with 17-AAG (0.25 μM) or PL (10 μM) or their combination for 2 h (**p < 0.01). Scale bar = 75 µm. C Representative images of cell trailing in a comet assay. Scale bar = 10 µm. D, E Representative images taken by fluorescence microscopy showing nuclear foci formation of γ-H2AX in HCT15 or RKO cells. Scale bar = 5 µm. F, G Representative images taken by fluorescence microscopy showing nuclear foci formation of 53BP1 in HCT116 or RKO cells. Scale bar = 5 µm.