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. 2023 Oct 13;9:375. doi: 10.1038/s41420-023-01672-y

Fig. 4. ROS scavenger reversed inhibition of cell viability by combination treatment.

Fig. 4

A–C Cell viability in HCT116, RKO, or HCT15 cells treated with the combination of 17-AAG and PL for 24 h following pretreatment with NAC (5 mM) for 1 h. D, E Colony-forming ability of HCT116, RKO, or HCT15 cells after pretreatment with NAC (5 mM). The concentration of 17-AAG on HCT116, RKO, and HCT15 cells was 0.0625 μM, 0.03125 μM and 1.25 μM, respectively, and the concentration of PL was 1.25 μM all the same. F Cell viability in HCT15 cells treated with the combination of 17-AAG and PL for 24 h following pretreatment with Z-VAD-FMK (20 μM) for 1 h. G, H The expression of cleaved-caspase-3 in HCT15 cells was detected by western blot (*p < 0.05, **p < 0.01).