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. 2023 Oct 13;9:375. doi: 10.1038/s41420-023-01672-y

Fig. 6. PL and 17-AAG synergize to induce ER stress in colon cancer cells.

Fig. 6

A–C The expression of GRP78, ATF4, and CHOP in HCT116, RKO, or HCT15 cells was detected by western blot. D–F The expression levels of ATF4 and CHOP in HCT116, RKO, or HCT15 cells. G, H Representative images showing the expression of CHOP in nuclear within HCT116 or RKO cells treated with 17-AAG or PL alone or their combination. Scale bar = 25 µm. I, J The expression of CHOP in HCT116 cells infected with recombinant lentivirus after 6 h of combination treatment. K The cell viability of HCT116 cells infected with recombinant lentivirus after 24 h of combination treatment was measured by trypan blue dye exclusion assay (**p < 0.01).