Fig. 7. ROS scavenger blocked the activation of ER stress.

A–C Cells were pretreated with NAC (5 mM) for 1 h before being treated with a combination of 17-AAG and PL, and the expression of ATF4 in HCT116, RKO, or HCT15 cells was detected by western blot (**p < 0.01). D–F The expression of CHOP in HCT116, RKO, or HCT15 cells. G, H Representative images showing expression of CHOP in nuclear within HCT116 or RKO cells were taken using a fluorescence microscope. Scale bar = 25 µm.