FIG. 8.

Effect of exposure to 17.27 μg of DS s3 (1-16) per ml on the efflux of UV-absorbing compounds from S. cerevisiae X2180-1A. The arrow and dotted line indicate when the peptide was added to the cultures. Efflux of UV-absorbing compounds was measured from cells resuspended in 25 mM citric-phosphate buffer at pH 4.5 (▴, ▵) and pH 6.0 (■, □). Efflux was determined in the presence of the peptide (open symbols) or without peptide (solid symbols) by measuring the optical density at 260 nm (adjusted for the presence of the peptide) and was expressed as the A₂₆₀ of the cell-free solution. A representative result is shown.