Table 10.

Effect of dietary treatment and period on jejunum, ileum, and colon abundance (AU) of high-mobility group box 1, claudin 3, and claudin 4

	Treatmenta				Period			P-value
Item	NC	BZA	BC50	AGP	1	2	SEMb	Treatment	Period	Treatment × period
Jejunumc
HMGB1	0.94	0.89	0.83	0.85	0.88	0.88	0.08	0.54	0.99	0.25
CLND3	1.20	1.37	1.22	1.10	1.22	1.23	0.17	0.54	0.98	0.92
CLDN4	1.50	1.62	1.98	1.84	2.13	1.33	0.28	0.52	0.003	0.78
Ileumd
HMGB1	1.13	0.98	1.11	0.99	1.15	0.95	0.10	0.45	0.02	0.38
CLND3	1.04	1.10	1.37	1.12	0.85	1.47	0.18	0.59	< 0.001	0.85
CLDN4	1.66	1.79	2.15	1.78	1.97	1.72	0.41	0.66	0.40	0.74
Colone
HMGB1	1.13	1.06	1.07	1.12	1.21	0.98	0.12	0.96	0.04	0.61
CLND3	1.66	1.08	1.29	1.92	0.97	2.00	0.50	0.50	0.02	0.51
CLDN4	1.79	1.63	1.43	1.01	0.95	1.97	0.72	0.83	0.09	0.87

^aPigs were fed experimental diets at weaning (28 d age) for 21 d. Treatments: NC: negative control (no additive); BZA: benzoic acid (0.60%); BC50: Benzocal-50 (0.20%); AGP: antibiotic growth promoter (Carbadox, 50 ppm). Period: period 1 = 7 d; period 2 = 14 d.

^bMaximum value for the standard error of the mean.

^cRepresentative blot images for jejunum CLDN3, CLDN4, and HMGB1 are provided in Supplementary Figure S1.

^dRepresentative blot images for ileum CLDN3, CLDN4, and HMGB1 are provided in Supplementary Figure S2.

^eRepresentative blot images for colon CLDN3, CLDN4, and HMGB1 are provided in Supplementary Figure S3.

^*,†Means without a common superscript differ after Tukey multiple comparison test, P < 0.05.