Table 1.

Primers used for the amplification of tgr5 gene of hybrid grouper

Gene	Primer sequence (5’–3’)	Usage
tgr5-PF	ATGATGGACTGCAACGACTCC	Partial PCR
tgr5-PR	GGTACCCAGCATGCCAGAAA
tgr5 5’	AGTTGGTCGTGTTGTG	RACE-PCR
	ATGATGACAAGGTTGGCC
tgr5 3’	ACATTTGTCGCCTCCATCGCTCAG
	CAGAGGCTGAACCTGCGGTACACT
tgr5-FF	GGAGGAAGTCGGCAGATT	Full-length PCR
tgr5-FR	CGGGTATCAACCACCATCAT