Table 2.
Primers designed for qPCR
| Target | Sequences forward | Sequences reverse | Primer efficiency (%) |
|---|---|---|---|
| tgr5 | ATGCCATCACCATACCGCTG | CCAGGCGATGCCTAAGATGA | 99.39 |
| tnfα | GCTGCGGCTCGAAGACAAT | CAGACGGTGCGGATGGAGT | 96.35 |
| il6 | GCACTACAAAATCTCCTCACTTCCA | GACCAGGAGGCCACCGTAGTA | 97.67 |
| il1β | CCAGCGTTGAGGGCAGAA | ATCGTCTCCAGATGTAAGGTT | 92.45 |
| p38a | AACTGGATGCACTACAACATGACA | TCCTGCTTATGAGGGAGGCTGGGG | 94.76 |
| p38b | ATCCTGGACTTTGGTTTGGCACGG | GAGATTTTCATCAAGAGCTCGGGC | 101.67 |
| p38c | CAGAGACCTCAAGCCAAGTAATGT | TCGATGTAGTCAGTTCCAGGAAAG | 91.82 |
| p53 | CGCAACAGGCTTCAATCGT | GAAGCATCAGAGGCGAAGA | 94.73 |
| pi3k-rs5 | GCCGAGGAGGAAGAGGATGTAGAC | GAGGAGATGGTGGAGAAGGTGGAG | 96.65 |
| 3-pdk1 | GGCAGCCATTACTGGAGCTTCTC | TGCGAGCAGGAACAGATGACAAC | 93.69 |
| tsc1 | GGACACCAAGGTCCAGAGCA | CGTCTCGGTCTGAAGCGTCT | 96.33 |
| tsc2 | TACGGAGACGACGGAGAGTTCAC | GGCATCAGTGTGGCTATGTGGAAG | 104.26 |
| akt | GGCAGGATGTGGTACAGAAGAAGC | TGTCTGGAGGAGTGAGTGTGATGG | 100.56 |
| ikka1 | GCAGAGGCAGCAGTGATG | CGCTCATTCTCGTCCAGTAAC | 98.22 |
| ikka2 | ACTGTTGGAGCCGATGGA | TGTGGACGACCTTCATACTCA | 94.92 |
| ikkb | GCCTTGGAGCCTCATGGACT | CGGTTTGGACGAAGCGGATG | 93.30 |
| ikbα1 | ACGCAGAACAGCCAGCAGCACAT | CGTGAAGCCGCCGTAGTTCAAGC | 94.69 |
| ikbα2 | CTCACCTACGGTCGCACCAA | GTCAGGCAACTCCCTCAGGT | 98.35 |
| p65 | AACCTCACCGAGCCCATTA | TTGTCACTCAGCCTGTATTCATCT | 101.83 |