Table 1:

Molecular Components of the PLVAC

Protein Name/gene ID	Known Function	Localization	Membrane/luminal	Phenotype (Fitness Score)	References
Cathepsin Protease L (TGME49_321530)	Cysteine Protease	PLVAC	Luminal	Dispensable in tachyzoites (0.68)/Significant in bradyzoites	(Parussini et al. 2010, Dou and Carruthers 2011)
Cathepsin Protease B (TGME49_249670)	Cysteine protease	PLVAC	Luminal	Dispensable (0.99)	(Que et al. 2002, Dou and Carruthers 2011)
Aspartyl Protease 1 (TGME49_201840)	Aspartic Peptidase family	PLVAC	Luminal	Dispensable (-0.36)	(McDonald et al. 2020)
Vacuolar Pyrophosphatase (TGME49_248670)	V-type H+-translocating pyrophosphatase VP1	PLVAC, PM, LE compartment, acidocalcisomes	Membrane	Dispensable (1.27)	(Miranda et al. 2010) (Liu et al. 2014)
Aquaporin1 (TGME49_215450)	Aquaporin water channel	PLVAC, PM	Membrane	Dispensable (0.84)	(Miranda et al. 2010)
Chloroquine Resistance Transporter (TGME49_313930)	CRT-like Chloroquine resistance transporter like	PLVAC	Membrane	Mild in tachyzoites/Moderate in bradyzoites (0.96)	(Warring et al. 2014, Kannan et al. 2019) (Thornton et al. 2019)
NHE3 (TGME49_305180)	Na+/H+ Exchanger Cation/H+ exchanger	PLVAC, LE	Membrane	Dispensable (0.0)	(Francia et al. 2011)
V-H + -ATPase (multiple gene IDs)	Vacuolar-H+-ATPase Multi-subunit	PLVAC, PM, pre-rhoptries	Membrane	Essential	(Stasic et al. 2019)
Zinc Transporter (TgZnT) (TGME49_251630)	Vacuolar Zinc Transporter	PLVAC, acidocalcisomes	Membrane	Growth defect of mutants (-3.06)	(Chasen et al. 2019)
Pi-Na+ Transporter (TGME49_240210)	Phosphate transporter, PiT family	PLVAC, cytoplasmic vesicles, PM	Membrane	Mild growth defect of mutants. Lower virulence (-1.32)	(Asady et al. 2020)
Vacuolar Iron Transporter (TgVIT) (TGME49_266800)	Vacuolar Iron Transporter	PLVAC, other unknown vesicles	Membrane	Mild growth defect of mutants. Lower virulence (-1.22)	(Aghabi 2022)