Skip to main content
. 2023 Oct 14;14:6489. doi: 10.1038/s41467-023-42322-2

Fig. 5. Loss of KDM2B-CxxC results in activation of the Wnt signaling pathway in the hippocampus.

Fig. 5

a The volcano plot of genes upregulated (red) and down-regulated (blue) in P0 Kdm2bEmx1-ΔCxxC hippocampi compared to controls, analyzed using DESeq2 (version 1.38.1). b, c GO analysis of the biological process of upregulated (b) and down-regulated (c) genes in P0 Kdm2bEmx1-ΔCxxC hippocampi, revealed terms related to cell proliferation (blue) and Wnt signaling pathways (red). d, e GSEA analysis of positive regulation of cell population proliferation (d) and Wnt signaling pathway (e). f The heat map of leading genes in (e). g, h X-gal staining on P0 coronal sections of Control_BAT (g) and Kdm2bEmx1-ΔCxxC_BAT (h) brains. Boxed regions were enlarged on the right (g’ and h’). Red arrows indicate areas where X-gal signals are significantly enhanced. i Quantification of normalized X-gal signal density on the DG-CA-FDJ-Sub path. n = 4 for Control_BAT brains and n = 3 for Kdm2bEmx1-ΔCxxC_BAT brains. j, k In situ hybridization (ISH) of Lef1 on E16.5 Control (j) and Kdm2bEmx1-ΔCxxC (k) coronal brain sections, with boxed regions magnified on the right (j’ and k’). Red arrows indicate areas where the Lef1 expression is significantly elevated. l Quantification of normalized ISH signal density of Lef1 on the DNE-HNE-Sub path. n = 4 for control brains and n = 4 for Kdm2bEmx1-ΔCxxC brains. m, n ISH of Sfrp2 on E16.5 control (m) and Kdm2bEmx1-ΔCxxC (n) coronal brain sections, with boxed regions magnified on the right (m’ and n’). o Quantification of normalized ISH signal density of Sfrp2 on the DNE-HNE-Sub path. n = 4 for control brains and n = 4 for Kdm2bEmx1-ΔCxxC brains. Gene Ontology (GO) analysis and Gene Set Enrichment Analysis (GSEA) in (b–e) were performed using clusterProfiler (version 4.2.2). Data are represented as means ± SEM. Statistical significance was determined using two-way ANOVA multiple comparisons test (i, l, o). Scale bars, 1 mm (g, h, j, k, m and n), 300 μm (g’, h’, j’, k’, m’ and n’). HP Hippocampus, DG dentate gyrus, FDJ fimbriodentate junction, CA Cornu Ammonis, Sub Subiculum, DNE dentate neuroepithelium, HNE hippocampal neuroepithelium, Ctx cortex, SVZ subependymal ventricular zone.