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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Biol Psychiatry. 2023 Apr 15;94(11):863–874. doi: 10.1016/j.biopsych.2023.04.006

Figure 5. Ethanol triggers internalization of GIRK channels and GABABR.

Figure 5.

A. Distribution of GIRK2 immunoparticles at the plasma membrane (PM; t6=14.19, ****P<0.0001; N=4/group; unpaired Student’s t-test) and intracellular sites (intra; t6=5.221, **P=0.002; N=4/group; unpaired Student’s t-test) in BA principal neurons from air- and ethanol-exposed mice. Small squares and circles represent individual data points from male and female subjects, respectively.

B. Distribution of GABABR1 immunoparticles at the plasma membrane (PM; t6=8.078, ***P=0.0002; N=4/group; unpaired Student’s t-test) and intracellular sites (intra; t6=12.74, ****P<0.0001; N=4/group; unpaired Student’s t-test) in BA principal neurons from air- and ethanol-exposed mice.

C. Plasma membrane-associated immunoparticle density for GIRK2 in spines (t6=7.566, ***P=0.0003; N=4/group; unpaired Student’s t-test) and dendrites (t6=2.892, *P=0.0276; N=4/group; unpaired Student’s t-test) from BA principal neurons from air- and ethanol-exposed mice.

D. Plasma membrane-associated immunoparticle density for GABABR1 in spines (t6=2.529, *P=0.0447; N=4/group; unpaired Student’s t-test) and dendrites (t6=3.916, **P=0.0078; N=4/group; unpaired Student’s t-test) from BA principal neurons from air- and ethanol-exposed mice.

E. Electron micrographs showing immunoparticles for GIRK2 (panels 1&2) and GABABR1 (panels 5&6) in the BA of air-exposed mice, and for GIRK2 (panels 3&4) and GABABR1 (panels 7&8) in ethanol-exposed mice. In sections from air-exposed mice, most immunoparticles for GIRK2 and GABABR1 were located along the extrasynaptic plasma membrane (arrows) of dendritic shafts (Den) and spines (s) of BA principal neurons. GIRK2 and GABABR1 immunoparticles were also detected at intracellular sites (crossed arrows). In sections from ethanol-exposed mice, GIRK2 and GABABR1 immunoparticles were commonly located at intracellular sites (crossed arrows), and less frequently along the extrasynaptic plasma membrane (arrows) of dendritic shafts (Den) and spines (s) of BA principal neurons. at, axon terminal. Scale bars: 500 nm.