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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Biol Psychiatry. 2023 Apr 15;94(11):863–874. doi: 10.1016/j.biopsych.2023.04.006

Figure 7. GIRK channel ablation in BA principal neurons recapitulates some ethanol-induced mood-related behavioral deficits.

Figure 7.

A. Depiction of viral targeting of the BA of Girk1fl/fl mice using AAV8-CaMKIIα-Cre(mCherry) or control (AAV8-CaMKIIα-mCherry) vector; the image below shows selective targeting of the BA in a mouse treated with the control vector.

B. Somatodendritic currents (Vhold=−60 mV) evoked by baclofen (200 μM) and reversed by CGP54626 (2 μM) in BA principal neurons from Girk1fl/fl mice, measured 5 wk after treatment with intra-BA AAV8-CaMKIIα-Cre(mCherry) or control (AAV8-CaMKIIα-mCherry) vector.

C. Ibaclofen in BA principal neurons from male Girk1fl/fl mice, measured 4–6 wk after intra-BA treatment with AAV8-CaMKIIα-Cre(mCherry) or AAV8-CaMKIIα-mCherry vector (t14=5.551, ****P<0.0001; N=8/group; unpaired Student’s t test).

D. Rheobase in BA principal neurons from male Girk1fl/fl mice treated with AAV8-CaMKIIα-Cre(mCherry) or AAV8-CaMKIIα-mCherry vector (t15=2.503, *P=0.0243; N=7–10/group; unpaired Student’s t test).

E. RMP in BA principal neurons from male Girk1fl/fl mice treated with AAV8-CaMKIIα-Cre(mCherry) or AAV8-CaMKIIα-mCherry vector (t6.771=0.792, P=0.455; N=7–10/group; unpaired Student’s t-test with Welch’s correction).

F. Handling-induced convulsion (HIC) scores of ethanol-naïve Girkfl/fl mice, assessed 5–6 wk after intra-BA treatment with AAV8-CaMKIIα-Cre(mCherry) or AAV8-CaMKIIα-mCherry vector (t29=1.397, P=0.173, N=12–19/group; unpaired Student’s t test), measured on Day 1.

G. Percentage of time spent in the light side of the light-dark box by Girkfl/fl mice treated with AAV8-CaMKIIα-Cre(mCherry) or control (AAV8-CaMKIIα-mCherry) vector (t26=0.841, P=0.408, N=11–17mice/group; unpaired Student’s t test), measured on Day 2.

H. Number of marbles buried by Girkfl/fl mice treated with AAV8-CaMKIIα-Cre(mCherry) or control (AAV8-CaMKIIα-mCherry) vector, measured on Day 4. A significant interaction was observed between sex and viral treatment (F(1,26)=6.377, P=0.0180; 2-way ANOVA); Sidak’s post hoc test revealed a viral treatment effect in females (**P=0.0071, N=6–8 mice/group) but not males (P=0.9453, N=6–10 mice/group).

I. Total irritability-like responses of Girkfl/fl mice treated with AAV8-CaMKIIα-Cre(mCherry) or control (AAV8-CaMKIIα-mCherry) vector (t29=6.319, ****P<0.0001, N=12–19 mice/group; unpaired Student’s t test), measured on Days 6 and 7.