| Antibody |
Rabbit anti-GIRK2 |
Frontier Institute Co. Japan |
Rb-Af290 |
aa. 390–421 of mouse GIRK2A-1; RRID: AB_2571712 |
| Mouse anti-GABABR1 |
Neuromab, CA, USA |
clone N93A/49 |
|
| Goat anti-rabbit IgG |
Nanoprobes Inc |
#2003 |
coupled to 1.4 nm gold |
| Goat anti-mouse IgG |
Nanoprobes Inc |
#2001 |
coupled to 1.4 nm gold |
| Bacterial or Viral Strain |
AAV8-CaMKIIa-mCherry |
University of Minnesota Viral Vector and Cloning Core |
|
Custom made from pAAV-CaMKIIα-hChR2(C128S/D156A)-mCherry (Addgene plasmid #35502, a gift from Dr. Karl Deisseroth) |
| AAV8-CaMKIIa-Cre-mCherry |
University of North Carolina Vector Core |
|
|
| Chemical Compound or Drug |
Baclofen |
Sigma |
B5399 |
|
| Barium Chloride |
Sigma |
B0750 |
|
| CGP54626 |
Tocris |
1088 |
|
| Pyrazole |
Sigma |
P56607 |
|
| Ethanol |
Pharmco |
111000200 |
|
| Commercial Assay Or Kit |
EnzyChromeTM ethanol assay kit |
BioAssay Systems, USA |
ECET-100 |
|
| Organism/Strain |
C57BL/6J |
The Jackson Laboratory |
Strain #:000664 |
|
| Girk1−/− |
PMID: 12374786 |
|
|
| Girk2−/− |
PMID: 9023358 |
|
|
| Girk3−/− |
PMID: 12040038 |
|
|
| Girk1flox/flox |
PMID: 28131769 |
|
|
| Sequence-Based Reagent |
CaMKIIa RNAScope probe |
Advanced Cell Diagnostics |
445231-C1 |
NM_009792.3; target nt region, 896–1986 |
| GIRK1 RNAScope probe |
Advanced Cell Diagnostics |
523951-C2 |
NM_008426.2; target nt region, 658–1679 |
| GIRK2 RNAScope probe |
Advanced Cell Diagnostics |
472321-C2 |
NM_001025584.2; target nt region, 282–1456 |
| GIRK3 RNAScope probe |
Advanced Cell Diagnostics |
508871-C2 |
NM_008429.2; target nt region, 84–1276 |
| Software; Algorithm |
Prism v.8 |
GraphPad Software, Inc |
|
|
