|
| Antibodies |
|
| Mouse anti-human CD66β (clone G10F5), Pacific Blue |
BioLegend |
RRID:AB_2563294 |
| Mouse anti-human CD3 (clone UCHT1), Alexa Fluor 700 |
BD Biosciences |
RRID:AB_396952 |
| Mouse anti-human CD107α (clone H4A3), PE-Cy5 |
BD Biosciences |
RRID:AB_396136 |
| Mouse anti-human IFNγ (clone B27), FITC |
BD Biosciences |
RRID: AB_398580 |
| Mouse anti-human MIP-1β (clone D21–1351), PE |
BD Biosciences |
RRID: AB_393549 |
| Mouse anti-human CD56 (clone B159), PE-Cy7 |
BD Biosciences |
RRID:AB_396853 |
| Mouse anti-human CD14 (clone MφP9), APC-Cy7 |
BD Biosciences |
RRID:AB_396889 |
| Mouse anti-human CD16 (clone 3G8), APC-Cy7 |
BD Biosciences |
RRID:AB_396864 |
| Anti-guinea pig complement C3 goat IgG fraction, FITC |
MP Biomedicals |
RRID:AB_2334913 |
| Anti-human IgG Fc antibody |
Jackson ImmunoResearch |
RRID: AB_2888996 |
| Mouse anti-rhesus IgG1 (clone 7H11) |
NHP Resource Center |
RRID:AB_2819310 |
| Mouse anti-rhesus IgG2 (clone 2C10) |
NHP Resource Center |
PR-3100 |
| Mouse anti-rhesus IgG3 (clone 2G11) |
NHP Resource Center |
PR-1130 |
| VIC panel of mAbs |
Saphire et al.13,17
|
N/A |
|
| Bacterial and virus strains |
|
| Ebola Zaire Kikwit (EBOV R4415, a 7U variant) |
Kugelman et al.59
|
N/A |
|
| Biological samples |
|
| Recombinant Vesicular Stomatitis Virus △G- Zaire Ebola glycoprotein vaccine |
Merck & Co |
N/A |
| LowTox Guinea Pig Complement |
CedarLane Labs |
Cat# CL4051 |
|
| Chemicals, peptides, and recombinant proteins |
|
| LC-LC-Sulfo-NHS Biotin |
ThermoFisher |
Cat# A35358 |
| Brefeldin A |
Sigma Aldrich |
Cat# B7651 |
| GolgiStop |
BD Biosciences |
Cat# 554724 |
| EBOV GP△TM |
Laboratory of E.O. Saphire |
N/A |
| Recombinant EBOV Soluble GP (sGP) |
Laboratory of E.O. Saphire |
N/A |
| Recombinant FcγRs (human and rhesus) |
Duke University Human Vaccine Institute |
N/A |
| Streptavidin-R-Phycoerythrin |
Prozyme |
Cat# PJ31S |
|
| Critical commercial assays |
|
| RosetteSep NK cell enrichment kit |
Stem Cell Technologies |
Cat# 15025 |
|
| Experimental models: Cell lines |
|
| THP-1 monocytes |
ATCC |
RRID: CVCL_0006 |
| VeroE6 |
ATCC |
RRID: CVCL_0574 |
|
| Experimental models: Organisms/strains |
|
| Cynomolgus macaques (Mauritius) |
New Iberia Research Center |
N/A |
| BALB/c mice |
Charles River Laboratory |
N/A |
|
| Software and algorithms |
|
| GraphPad Prism 8 |
GraphPad Software, Inc. |
RRID:SCR_002798 |
| R Studio |
R Project for Statistical Computing |
RRID:SCR_000432 |
| Flow Jo |
BD Bioscience |
RRID:SCR_008520 |
| JMP Pro 15 |
JMP |
RRID:SCR_014242 |
| MATLAB ver. R2019a |
MathWorks |
RRID:SCR_001622 |
|
| Other |
|
| FluoSpheres® NeutrAvidin®-Labeled Microspheres, 1.0 μm, yellow-green fluorescent (505/515), 1% solids |
Life Technologies |
Cat# F-8776 |
| FluoSpheres® NeutrAvidin®-Labeled Microspheres, 1.0 μm, red fluorescent (580/605), 1% solids |
Life Technologies |
Cat# F8775 |
| MagPlex microspheres |
Luminex corporation |
Cat# MC12001 –01, MCI12040–01, MCI10077–01 |
