TABLE 1.
Bacterial strains and plasmids used
| E. coli strain or plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| TUM1083 | Clinical isolate, cefotaxime-resistant strain | This study |
| TUM1103 | Transformant, E. coli AS226 harboring pMTY036 | This study |
| ML4909 | F−galK2 galT22 hsdR metB1 relA supE44 Rifr | M. Inoue |
| C600 | F−thi-1 thr-2 leuB6 lacY1 tonA21 supE44 λ−Rifr | 27 |
| AS226 | F−thr-1 leuB6 thi-1 hsdS1 lacY1 tonA21 supE44 ampCΔ λ− | 13 |
| MV1184 | ara Δ (lac-proAB) Δ (srl-racA) 306::Tn10 φ80 dlacZ ΔM15 rpsL thi [F′ lacIqlacZ ΔM15proAB traD36] | 31 |
| Plasmids | ||
| pHSG397 | lac cat | 30 |
| pMTY002 | Cefotaxime resistant | This study |
| pMTY036 | 2.1-kb Sau3AI fragment from pMTY002 cloned into pHSG397 | This study |
| R388 | IncW | 33 |
| R386 | IncFI | 27 |
| R100-1 | IncFII | 27 |
| R124 | IncFIV | 27 |
| R64-11 | IncIα | 27 |
| R621α | IncIγ | 10 |
| R27 | IncHI | 27 |
| N3 | IncN | 27 |
| R751 | IncP | 15 |
| R446B | IncM | 27 |
| R401 | IncT | 27 |
| R6K | IncX | 27 |