Fig. 6.

GSK-J4 impaired in vivo microtumor formation of 22Rv1-RR cells. a Representative images of CAM microtumors for 22Rv1-P, RR, RR vehicle (DMSO), and RR GSK-J4-treated cells with or without expsure to 2.5 Gy of IR-SD. Digital images were taken under a stereomicroscope Olympus S2X16 using a digital camera Olympus SC180. b Relative area (pixel²) of the formed microtumors was assessed using ImageJ software tools. Results are presented as mean ± SD of ten eggs per group condition; *P < 0.05; **P < 0.01; ****P < 0.001. c Representative IHC images for p53, KDM6B, and H3K27me3 protein expression in CAM-assay microtumors tissue sections for both groups of vehicle and GSK-J4. Pictures were taken using Olympus BX41 microscope with a digital camera Olympus U-TV0.63XC in CellSens software (version V0116, Olympus), at ×400 magnification (scale bar, 20 μm). d p53, e KDM6B, and f H3K27me3—% of positive staining cells in vehicle and GSK-J4 treatment groups. Scatter plot bar graphs depict mean ± SD of all selected microtumors. *P value < 0.05. CAM Chicken choriallantoic membrane, P parental, PCa prostate cancer, RR radioresistant, SD single-dose