Skip to main content
. 2023 Aug 8;109(4):552–565. doi: 10.1093/biolre/ioad091

Figure 2.

Figure 2

Effect of phthalate mixture exposure on P4 levels and PGR mRNA levels. Human granulosa cells from in vitro fertilization patients were treated with DMSO (vehicle control) or phthalate mixture (PHTmix; 1–500 μg/ml) for 48 h prior to hCG treatment. Following 48 h, the cells were treated with or without hCG and with or without PHTmix. Media were collected at multiple time-points following hCG treatment and were subjected to progesterone measurements via an Immulite kit (A). Cells were collected at multiple time-points following hCG treatment and were subjected to qPCR to measure PGR mRNA levels that were normalized to GAPDH (B). Values are presented as a percent fold-change relative to the hCG alone control group for each time-point collected. Graphs represent mean ± SEM. Bars that do not share a letter designation are significantly different (n = 9–12 patients/group, p ≤ 0.05).