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. 2023 Aug 8;109(4):552–565. doi: 10.1093/biolre/ioad091

Figure 6.

Figure 6

Effect of progestin supplementation on phthalate mixture-induced toxicity. Human granulosa cells from in vitro fertilization patients were treated with DMSO (vehicle control) or phthalate mixture (PHTmix; 500 μg/ml) for 48 h prior to hCG and progestin (R5020) treatment. Following 48 h, the cells were treated with or without hCG, with or without PHTmix, and with or without R5020 (100–1000 ng/ml). Media were collected at multiple time-points following hCG treatment and were subjected to progesterone measurements via an Immulite kit (A, B). Cells were collected at multiple time-points following hCG treatment and were subjected to qPCR to measure the mRNA levels of PGR (C) and RGS2 (D, E) that were normalized to GAPDH. Values are presented as a percent fold-change relative to the hCG alone control group for each time-point collected. Graphs represent mean ± SEM. Bars that do not share a letter designation are significantly different (n = 4–8 patients/group, p ≤ 0.05).