Skip to main content
. 2023 Aug 8;109(4):552–565. doi: 10.1093/biolre/ioad091

Figure 7.

Figure 7

Effect of cAMP supplementation on phthalate mixture-induced decreases in P4 levels and PGR mRNA levels. Human granulosa cells from in vitro fertilization patients were treated with DMSO (vehicle control) or phthalate mixture (PHTmix; 500 μg/ml) for 48 h prior to hCG and 8-Br-cAMP (cAMP) treatment. Following 48 h, the cells were treated with or without hCG, with or without PHTmix, and with or without cAMP (1.5 mM). Media were collected at multiple time-points following hCG treatment and were subjected to progesterone measurements via an Immulite kit (A). Cells were collected at multiple time-points following hCG treatment and were subjected to qPCR to measure PGR mRNA levels that were normalized to GAPDH (B). Values are presented as a percent fold-change relative to the hCG alone control group for each time-point collected. Graphs represent mean ± SEM. Bars that do not share a letter designation are significantly different (n = 4–8 patients/group, p ≤ 0.05).