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Effect of treatment on the histotroph metabolome from lactating dairy cows on day 16 of pregnancy presented as: volcano plot (A); principal component analysis (B); partial least squares—discriminate analysis (C); 15 affected metabolites by treatment based on the VIP score (D); and heatmap of the 50 metabolites discriminating ENDO from CON treatment based on the VIP score (E). The histotroph was collected using the cytobrush technique on day 16 of pregnancy from 8 CON and 12 ENDO cows.
