Table 1.
Effect of treatment on endometrial polymorphonuclear cells, incidence of subclinical endometritis, rectal temperature, and conceptus development in lactating Holstein cows receiving AI
| Item | Treatment1 | |||
|---|---|---|---|---|
| CON | ENDO | SEM | P-value | |
| Polymorphonuclear cells,2 % | ||||
| Day 2 | 5.5 | 22.7 | 1.7 | <0.001 |
| Day 7 | 12.2 | 17.0 | 1.9 | 0.07 |
| Subclinical endometritis,2,3 % | ||||
| Day 2 | 14.3 | 88.0 | 6.8 | <0.001 |
| Day 7 | 30.0 | 73.3 | 10.2 | 0.008 |
| Rectal temperature, °C | 38.46 | 38.49 | 0.06 | 0.63 |
| Serum haptoglobin, μg/mL | 179.8 | 195.7 | 27.8 | 0.70 |
| Synchronized ovulation, % | 95.0 | 82.4 | 5.7 | 0.22 |
| Pregnant day 16,4 % | ||||
| All cows | 42.3 | 48.3 | 10.3 | 0.68 |
| Synchronized cows | 59.7 | 60.9 | 14.5 | 0.95 |
| Conceptus length, cm | 13.5 | 8.5 | 1.5 | 0.03 |
| Interferon-tau in uterine flush, ng/mL | 262.7 | 56.6 | 67.6 | 0.04 |
1Holstein cows at 26 ± 3 days postpartum were blocked by parity and genomic breeding value for cow conception rate, and, within block, they were assigned randomly to remain as controls (CON; n = 23) or to receive an intrauterine infusion of 5.20 × 108 CFU E. coli and 4.16 × 108 CFU T. pyogenes during the luteal phase to induce endometrial inflammation (ENDO; n = 34). Conceptuses recovered on day 16 of pregnancy from 8 CON and 12 ENDO cows.
2Interaction between treatment and day after enrollment (P < 0.001).
3Based on >10% of polymorphonuclear cells in endometrial cytology.
4Pregnancy per AI on day 16 after insemination based on interferon-tau in the uterine flush fluid.