TABLE 1.

(A) Summary of variants identified by sequencing of 9p21.3 in 450 centenarians and 500 controls arranged by minor allele frequency ranges. Variant annotation was performed with dbSNP database 149. (B) Functional annotation of 1064 identified variants with at least four allele counts. Genes considered include CDKN2A, CDKN2B, and noncoding transcript ANRIL (CDKN2BAS1). (C) List of top 10 longevity‐associated variants.

(A)
MAF Range	SNP: N=2056		Complex: N=160
	Known variants	Novel variants	Known variants	Novel variants
0%–0.5%	557	664	39	47
0.5%–1%	109	29	3	3
1%–5%	259	21	20	11
5%–10%	93	0	10	5
10%–25%	99	0	11	5
25%–50%	225	0	6	0

(B)
	Total	SNP		Indels
		Known Variants	Novel Variants	Known Variants	Novel Variants
Genic	765	597	106	36	26
Exonic	45	33	9	2	1
Synonymous	1	1	0	0	0
Nonsynonymous	7	4	3	0	0
Noncoding RNA	21	16	4	1	0
5' UTR	2	1	1	0	0
3' UTR	14	11	1	1	1
Intronic	720	564	97	34	25
Splicing	2	2	0	0	0
Intergenic	526	436	51	28	11

(C)
Top longevity variants	Chr9 position (hg19)	Allele	MAF (case)	MAF (con)	p Value	Function
rs74605971	21,947,885	T > A	0.052	0.095	0.00044	Intergenic
rs12335941	21,955,669	G > A	0.339	0.413	0.00091	Intergenic
rs11521166	21,948,376	C > T	0.053	0.092	0.00147	Intergenic
rs111310495	21,990,187	A > G	0.002	0.016	0.00158	Intronic
Novel	22,068,072	G > A	0.002	0.016	0.00158	ncRNA_intronic
rs10757261	21,954,953	A > G	0.351	0.422	0.00159	Intergenic
rs2106118	21,949,528	T > A	0.401	0.473	0.00165	Intergenic
rs2106117	21,949,527	T > G	0.402	0.472	0.00226	Intergenic
rs10738612	22,174,103	T > C	0.438	0.506	0.00323	Intergenic
rs2518722	21,952,926	C > T	0.244	0.304	0.00398	Intergenic