TABLE 2.
Conventional cell lines and primary cells used to screen for senolytic activity.
| Cell name | Morphology | Tissue of origin | Reasons for use | Screening examples |
|---|---|---|---|---|
| WI‐38 | Fibroblast | Human lung | First cell line used to study replicative senescence, well‐characterized senescence phenotype and established senescence induction methods | Chang et al. (2016); He, Li, et al. (2020); Li et al. (2019); Moaddel et al. (2022); Wang et al. (2016) |
| IMR‐90 | Fibroblast | Human lung | Similar properties to WI‐38 and commonly used as an alternative cell line | Guerrero et al. (2019); Wakita et al. (2020); Yosef et al. (2016); Zhu et al. (2017) |
| Human umbilical vein endothelial cell (HUVEC) | Endothelial | Vein of human umbilical cord | Well‐established senescence induction methods, cells senesce quickly following serial passage, established model for aging endothelial cells and vasculature | Kusumoto et al. (2021); Zhu et al. (2017); Zhu et al. (2015) |
| Human dermal fibroblasts (HDFs) | Fibroblast | Human skin | Well‐established senescence induction methods, used as a model for studying skin tissue dysfunction | Cho et al. (2020); Go et al. (2021); Kim et al. (2022); Lammermann et al. (2018) |
| BJ fibroblasts | Fibroblast | Human foreskin | Well‐established senescence induction methods, used as a cellular model of skin aging | Hubackova et al. (2019); Ozsvari et al. (2018) |
| Ercc1 −/− mouse embryonic fibroblasts | Fibroblast | Embryos of pregnant Ercc −/ Δ mice | Mutation in ERCC1 DNA repair protein is associated with a rapid aging phenotype, cells senesce quickly following exposure to atmospheric O2, used as an in vitro precursor to senolytic trials in the Ercc −/ Δ mouse model of human progeroid syndrome | Fuhrmann‐Stroissnigg et al. (2017); Yousefzadeh et al. (2018) |
| Preadipocytes | Fibroblast | Human adipose | Senescent preadipocytes are abundant in human tissue and contribute to impaired adipogenesis and age‐related metabolic dysfunction | Zhu et al. (2015) |
| Annulus fibrosus and nucleus pulposus cells | Fibroblast | Human intervertebral discs | Senescent cells from intervertebral discs contribute to inflammation and back pain | Cherif et al. (2019); Cherif et al. (2020) |
| Various cancer cell lines | Various | Various (breast, lung, brain, liver, mouth, pharynx) | Senescent cells are a feature of tumors and contribute to cancer progression and treatment resistance, common anti‐cancer therapies induce cellular senescence | Hubackova et al. (2019); Samaraweera et al. (2017); Triana‐Martinez et al. (2019); Troiani et al. (2022); Yang, Tian, et al. (2021) |